STEC Diagnostics

Shiga toxin-producing Escherichia coli (STEC) cause foodborne and waterborne diarrheal disease that can progress to hemorrhagic colitis and hemolytic uremic syndrome (HUS).

STEC derive their name from their ability to produce toxins (Stx1 & Stx2) similar in structure and function to the Shiga toxin produced by Shigella dysenteriae.

STEC strain O157:H7 has historically been the focus of attention in the United States, where it was first isolated from undercooked hamburgers. The strain causes an estimated 73,000 illnesses annually. STEC infections caused by non-O157 strains have become more prevalent in recent years, both in the U.S. and globally.

Since certain treatments and medications can increase the risk of HUS, prompt detection of STEC is necessary to prevent outbreaks and secondary transmission.

Our tests can detect STEC present in fecal samples or culture, regardless of the serotype or other virulence factors.

Microassay Plates


Product NameCatalog #Suggested CPT Code*Tests/KitAnalyte(s) DetectedFormatTime to ResultFecal Sample Type
25Shiga Toxin 1 and Shiga Toxin 2Rapid EIA< 30 minFresh fecal sample (unpreserved) / Cary Blair / C&S / Broth (GN or MAC) / Plate
Culture (SMAC,CT-SMAC,CHROMagar®O157)
SHIGA TOXIN CHEK T306968742796Shiga Toxin 1 and Shiga Toxin 2Microplate ELISA50 min, or 20 (rapid format)Fresh fecal sample (unpreserved) / Cary Blair / C&S / Broth (GN or MAC) / Plate
Culture (SMAC,CT-SMAC,CHROMagar®O157)
Additional Shiga Toxin resources:

Shiga Toxin Producers
by J. Heptinstall and A. Dandro, TECHLAB®

E. coli O104:H4 – The Outbreak Strain in Germany by D. Campbell, TECHLAB®