An enzyme immunoassay for the detection of glutamate dehydrogenase (GDH) produced by both toxigenic and non-toxigenic strains of Clostridium difficile.
The glutamate dehydrogenase (GDH) of C. difficile is a good antigen marker for the organism in feces because it is produced in high amounts by all strains, toxigenic or non-toxigenic. The antigen can be detected in fecal specimens by using the C. DIFF CHEK™ -60 test.
A positive result in the test, which is highly specific for the glutamate dehydrogenase of C. difficile, confirms the presence of this organism in a fecal specimen; a negative result indicates the absence of the organism. A positive result should be followed by a toxin-specific test to confirm the presence of toxigenic C. difficile.