T5033 - TOX A/B QUIK CHEK®
The TOX A/B QUIK CHEK® test is a rapid format that uses antibodies specific for toxins A and B of C. difficile. The device contains a Reaction Window with two stripes. One stripe is a test line specific for toxins A and B. The other stripe is the control line.
The fecal sample is added to Diluent and Conjugate. The mixture is transferred to the Sample Well and the device is incubated for 15 minutes. During this time, any toxin A and toxin B bind to the conjugate and the complexes are captured by the antibodies in the test line. The Reaction Window is washed and Substrate is added to read the reaction.
Proper test performance is indicated by the formation of a blue line at the control side (C) of the Reaction Window.
A positive result is indicated by the formation of a blue line at the test (T) side of the Reaction Window.
Features
- Patent pending technology
- Results in under 30 minutes
- Easy to interpret
- Highly sensitive and specific
Further Information
Package Insert Issued 2/07
CPT Codes Issued 3/08
Material Safety Data Sheet Issued 7/06
Tutorial
TOX A/B QUIK CHEK® [Macromedia Flash Player Required]
A step by step animated guide to detecting C. difficile toxins A and B in fecal specimens using the TOX A/B QUIK CHEK®.
Poster Presentation
Comparative Evaluation of Six Commercially Available Assays for the Detection of Clostridium difficile Toxins in Fecal Specimens
Poster Presentation
Comparison of the C. difficile TOX A/B QUIK CHEK® with commercial C. difficile A+B ELISAs
American Society for Microbiology meeting, May 2006, Orlando, FL
Poster Presentation
Comparison of the TOX A/B QUIK CHEK® to tissue culture assay and to a commercial A+B ELISA for the detection of Clostridium difficile toxins in fecal specimens
American Society for Microbiology meeting, May 2006, Orlando, FL
Poster Presentation
A New Rapid Test for Detecting Clostridium difficile Toxins A and B in Fecal Specimens
American Society for Microbiology meeting, June 2005, Atlanta, GA
Frequently Asked Questions
Q: Only one line is visible after running the test, what does that mean?
A: One visible line could mean two things. If the visible line is located on the side of the cassette labeled "C" (for Control Line), then the test ran properly and no C. difficile toxin was detected in the sample. If the visible line is located on the side of the cassette labeled "T" (for Test Line), then the test DID NOT run properly, the results are invalid and the sample should be tested again using a fresh cassette. Q: No line is visible after running the test, what does that mean?
A: No visible line means that the test DID NOT run properly. The sample should be tested again using a fresh cassette.
Q: The membrane surface appears dry, even though diluted sample has been added. What should I do?
A: After 5 minutes, if the membrane still appears to be dry, up to 100mL (4 drops) of Diluent can be added to the Sample Well to aid in the "wicking" process. An additional 5 minutes of incubation is required for a total of 20 minutes from the addition of sample. If within 5 minutes the additional Diluent is insufficient to cause wetting of the membrane in the Reaction Window, the test is invalid and the sample should be tested again using a fresh cassette.
Q: What is the CPT code for the TOX A/B QUIK CHEK®?
A: According to the 2006 coding, the CPT code is 87324.